LOCUS       sequence-98487-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for cloning and expression of FLAG-GFP tagged proteins 
            (C-terminal tag). Confers resistance to G418..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Britton S, Dernoncourt E, Delteil C, Froment C, Schiltz O, Salles B,
            Frit P, Calsou P
  TITLE     DNA damage triggers SAF-A and RNA biogenesis factors exclusion from 
            chromatin coupled to R-loops removal.
  JOURNAL   Nucleic Acids Res. 2014 Oct 1;42(14):9047-62. doi: 
            10.1093/nar/gku601. Epub 2014 Jul 16.
  PUBMED    25030905
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku601"; journalName: "Nucleic Acids Res"; date: 
            "2014-10-1- 1"; volume: "42"; issue: "14"; pages: "9047-62"
COMMENT     Sequence Label: pEGFP-N1-FLAG
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(29..52)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    71..127
                     /label=MCS
                     /note="multiple cloning site"
     promoter        complement(168..371)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(197..217)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        372..675
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccttgctcac catggtggcg accggtggct tatcgtcgtc atccttgtaa tcacgcgtca
       61 tggtggcggc ggatcccggg cccgcggtac cgtcgactgc agaattcgaa gcttgagctc
      121 gagatctgag tccggtagcg ctagcggatc tgacggttca ctaaaccagc tctgcttata
      181 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
      241 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
      301 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
      361 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
      421 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
      481 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
      541 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
      601 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
      661 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
      721 tactattaat aactaatgca tggcggtaat acggttatcc acagaatcag gggataacgc
      781 aggaaagaac atgtgagc
//
