LOCUS       sequence-98493-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yellow beetle luciferase with nuclear localization signal (NLS) and 
            ClpXP degron tag (ssrA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB775
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..58
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(143..161)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ccttcttagt cgctgaattt aattaaccat ggctccacca aaaaaaaaaa gaaaagttgc
       61 catgggtggg ggcagcatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
      121 tcctctagag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
      181 ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga
      241 atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
      301 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
      361 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
      421 caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa
      481 aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa
      541 aacggattac cagggatttc agtcgatgta tacgttcgtc acatctcatc tacctcccgg
      601 ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat
      661 aatgaattcc tctggatcta ctgggttacc taagg
//
