LOCUS       sequence-98495-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal 
            tag). Confers resistance to G418..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Britton S, Dernoncourt E, Delteil C, Froment C, Schiltz O, Salles B,
            Frit P, Calsou P
  TITLE     DNA damage triggers SAF-A and RNA biogenesis factors exclusion from 
            chromatin coupled to R-loops removal.
  JOURNAL   Nucleic Acids Res. 2014 Oct 1;42(14):9047-62. doi: 
            10.1093/nar/gku601. Epub 2014 Jul 16.
  PUBMED    25030905
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku601"; journalName: "Nucleic Acids Res"; date: 
            "2014-10-1- 1"; volume: "42"; issue: "14"; pages: "9047-62"
COMMENT     Sequence Label: pEGFP-N1-FUS/TLS-FLAGC
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(34..57)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcgaccgg tggcttatcg tcgtcatcct tgtaatcacg
       61 cgtatacggc ctctccctgc gatcctgtct gtgctcaccc ctggaatcca tcttgccagg
      121 gccaaagcca cctctgtccc caccaccccg gccccctcgg aagcctccac ggtccccgcc
      181 gcggccccgg tagccgcctc gatcatagcc tcctctgcca ccacgacgat catccccgta
      241 gttacccccc atgtgagagc cacctggtcc ccctcctggg ccatctggtt taggggcctt
      301 acactggttg cattcattcc tccaagagaa gttcatattc tcacaggtgg gattaggaca
      361 cttccagtca ccagctcgct gctgtcctcc accgccacca cctccactgg gaaatcctcc
      421 tcggccacca ccaccactgc caccacctcc atagcctcca cggcccatgg gtcctcctcg
      481 ccctcggcct ccacgaccat tgccaccacc ccgattaaag tctgcccggc gagtagcaaa
      541 tgagaccttg ataggatttc cggagaattc tttaccatca aaccagtcaa tagctgcttt
      601 agctgaaggt gggtcatcaa aagagaccgt tgcctctccc ttcagcttgc cagtttccct
      661 gtctgtgtac aaattaatca tgggctgtcc cgttttcttg tttgtcttaa taataccaat
      721 ctgcttgaag taatcagcca cagactcaat tgtaacattc tcacccaggc cttgcacaaa
      781 gatggtgttg ttgtctgaat tatcctgttc ggagtcatga cgtgatcctt ggtcccgagg
      841 gccaccaaat ttattgaagc caccacggtc acttccgccc atgccacctc tgcctccacg
      901 gc
//
