LOCUS       sequence-98508-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dre-responsive fluorescent reporter plasmid containing a 
            STOP-cassette flanked by Dre-recognition sites (rox) followed by 
            ZsGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-roxSTOProx-ZsGreen
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      685..694
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    702..733
                     /label=rox
                     /bound_moiety="Dre recombinase"
     primer_bind     complement(786..805)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aggcggagcc ggaggcgatg gcgtgctcgg tcaggtgcca cttctggttc ttggcgtcgc
       61 tgcggtcctc gcgggtcagc ttgtgctgga tgaagtgcca gtcgggcatc ttgcggggca
      121 cggacttggc cttgtacacg gtgtcgaact ggcagcgcaa gcggccaccg tccttcagca
      181 gcaggtacat gctcacgtcg cccttcaaga tgccctgctt gggcacgggg atgatcttct
      241 cgcaggaggg ctcccagttg tcggtcatct tcttcatcac ggggccgtcg gcggggaagt
      301 tcacgccgta gaacttggac tcgtggtaca tgcagttctc ctccacgctc acggtgatgt
      361 cggcgttgca gatgcacacg gcgccgtcct cgaacaggaa ggagcggtcc caggtgtagc
      421 cggcggggca ggagttcttg aagtagtcga cgatgtcctg ggggtactcg gtgaacacgc
      481 ggttgccgta catgaaggcg gcggacaaga tgtcctcggc gaagggcaag gggccgccct
      541 ccaccacgca caggttgatg gcctgcttgc ccttgaaggg gtagccgatg ccctcgccgg
      601 tgatcacgaa cttgtggccg tccacgcagc cctccatgcg gtacttcatg gtcatctcct
      661 tggtcaggcc gtgcttggac tgggccatgg tggcggctgc ctaactttaa ataatgccaa
      721 ttatttaaag ttaattaagg gttccggatc agcttgatgg ggatccagac atgataagat
      781 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      841 aaa
//
