LOCUS       sequence-98511-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) and yeast codon-optimized yellow 
            fluorescent protein (yEVenus) fusion with PEST degron driven by 
            MET17 promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB682
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        94..443
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     444..460
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     444..460
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(543..561)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 ccggatgcaa gggttcgaat cccttagctc tcattatttt ttgctttttc tcttgaggtc
      121 acatgatcgc aaaatggcaa atggcacgtg aagctgtcga tattggggaa ctgtggtggt
      181 tggcaaatga ctaattaagt tagtcaaggc gccatcctca tgaaaactgt gtaacataat
      241 aaccgaagtg tcgaaaaggt ggcaccttgt ccaattgaac acgctcgatg aaaaaaataa
      301 gatatatata aggttaagta aagcgtctgt tagaaaggaa gtttttcctt tttcttgctc
      361 tcttgtcttt tcatctacta tttccttcgt gtaatacagg gtcgtcagat acatagatac
      421 aattctatta cccccatcca tactctagaa ctagtggatc ccccgggtta attaacatgg
      481 aagacgtcaa aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg
      541 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
      601 ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc
      661 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
      721 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
      781 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc
      841 agcctaccgt ggtgttcgtt tccaaaa
//
