LOCUS       sequence-98523-e        3269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template for generating gene 60 mRNA, coding gap deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60 delta gap
FEATURES             Location/Qualifiers
     source          1..3269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        429..447
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1048..1064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1048..1064)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1061..1083)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1072..1088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1096..1102,1103..1120,1121..1126))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1141..1162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1279..1296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1450..2038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1530..1549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2209..3000,3001..3069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2832..2851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3070..3174)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3242..3260
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagata atacgactca ctataggagc atttaatgaa tttaaacgcg gttggattcc
      481 tgatgaaaag ttctatgagg tgtataatga aatttgtaaa aattgattct tctagcgttg
      541 atatgaaaaa atataaattg cagaacaatg ttcgtcgttc tattaaatcc tcttcaatga
      601 actatgcgaa tgtcgctatt atgacagacg cagatcacga tggattaggt tctatttatc
      661 cttctctgct cggatttttt agtaattggc cagaattgtt tgagcaagga cgaattcgct
      721 ttgtcaaaac tcctgtaatc atcgctcagg tcggtaaaaa acaagaatgg ttttatacag
      781 tcgctgaata tgagagtgcc aaagatgctc tacctaaaca tagcatccgt tatattaagg
      841 gacttggctc tttggaaaaa tctgaatatc gtgagatgat tcaaaaccca gtatatgatg
      901 ttgttaaact tcctgagaac tggaaagagc tttttgaaat gctcatggga gataatgctg
      961 accttcgtaa agaatggatg agccagtagt ttactttacc acaaggatgt ggtataatta
     1021 attgggcaaa agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1081 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1141 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1201 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1261 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1321 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1381 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1861 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     1921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2521 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2821 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3061 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3181 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     3241 aaataggcgt atcacgaggc cctttcgtc
//
