LOCUS       sequence-98528-e        2930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template for generating gene 60 mRNA, sequence 5' of coding gap + 
            Cys.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60 to gap + Cys
FEATURES             Location/Qualifiers
     source          1..2930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        429..447
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(709..725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(709..725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(722..744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    733..749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(757..763,764..781,782..787))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    802..823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(940..957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1111..1699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1191..1210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1870..2661,2662..2730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2493..2512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2731..2835)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2903..2921
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagata atacgactca ctataggagc atttaatgaa tttaaacgcg gttggattcc
      481 tgatgaaaag ttctatgagg tgtataatga aatttgtaaa aattgattct tctagcgttg
      541 atatgaaaaa atataaattg cagaacaatg ttcgtcgttc tattaaatcc tcttcaatga
      601 actatgcgaa tgtcgctatt atgacagacg cagatcacga tggatgttag tttactttac
      661 cacaaggatg tggtataatt aattgggcaa aagcttggcg taatcatggt catagctgtt
      721 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      781 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
      841 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
      901 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      961 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1021 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1081 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1141 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1201 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1261 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1321 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1381 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1441 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1501 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     1561 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1621 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1681 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1741 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1801 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1861 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1921 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1981 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2041 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2101 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2161 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2221 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2281 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2341 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2401 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2461 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     2521 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2581 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2641 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2701 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2761 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2821 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     2881 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
