LOCUS       sequence-98529-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis 
            with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal 
            tyrosine (delta Y).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS426-TUB1-internal6xHis - human TUBA1a Cterm...
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        30..471
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    30..147
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     442..465
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     486..502
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     486..502
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             752..769
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcctaaggga caaagctgga gctctagtac ggattagaag ccgccgagcg ggtgacagcc
       61 ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc
      121 agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact agcttttatg
      181 gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat gaacgaatca
      241 aattaacaac cataggatga taatgcgatt agttttttag ccttatttct ggggtaatta
      301 atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa actgcataac
      361 cactttaact aatactttca acattttcgg tttgtattac ttcttattca aatgtaataa
      421 aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga gaaaaaaccc
      481 cggattctag aactagtgga tccaaaatga gagaagttat tagtattaat ggtatgttcg
      541 atttgcccgt ccaggctaga tctttttttt gttagttcat tttggcgttt catctttcat
      601 tactaacttc aacgcaaaat tttttttttt ttgatttctc tttacagtcg gtcaagctgg
      661 ttgtcagatt ggtaatgcct gttgggaatt atattccctt gagcacggta ttaagccgga
      721 tggacatcta gaagatggcc tttcaaagcc gcatcatcac catcatcaca agggaggaga
      781 agagggtttc tccacgtttt tccatgaaac cggctacggt aagttcgttc c
//
