LOCUS       sequence-98531-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yellow beetle luciferase with nuclear localization signal (NLS) and 
            ClpXP degron tag (ssrA), driven by MET17 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB690
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        94..443
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     444..460
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     444..460
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             487..507
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(592..610)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 ccggatgcaa gggttcgaat cccttagctc tcattatttt ttgctttttc tcttgaggtc
      121 acatgatcgc aaaatggcaa atggcacgtg aagctgtcga tattggggaa ctgtggtggt
      181 tggcaaatga ctaattaagt tagtcaaggc gccatcctca tgaaaactgt gtaacataat
      241 aaccgaagtg tcgaaaaggt ggcaccttgt ccaattgaac acgctcgatg aaaaaaataa
      301 gatatatata aggttaagta aagcgtctgt tagaaaggaa gtttttcctt tttcttgctc
      361 tcttgtcttt tcatctacta tttccttcgt gtaatacagg gtcgtcagat acatagatac
      421 aattctatta cccccatcca tactctagaa ctagtggatc ccccgggtta attaaccatg
      481 gctccaccaa aaaaaaaaag aaaagttgcc atgggtgggg gcagcatgga agacgccaaa
      541 aacataaaga aaggcccggc gccattctat cctctagagg atggaaccgc tggagagcaa
      601 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
      661 catatcgagg tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa
      721 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
      781 cttcaattct ttatgccggt gttgggcgcg ttatttatcg ga
//
