LOCUS       sequence-98532-e        2986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template for generating gene 60 mRNA truncated two codons after 
            coding gap + Cys.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60 thru gap + Cys
FEATURES             Location/Qualifiers
     source          1..2986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        429..447
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(765..781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(765..781)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(778..800)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    789..805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(813..819,820..837,838..843))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    858..879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(996..1013)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1167..1755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1247..1266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1926..2717,2718..2786))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2549..2568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2787..2891)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2959..2977
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagata atacgactca ctataggagc atttaatgaa tttaaacgcg gttggattcc
      481 tgatgaaaag ttctatgagg tgtataatga aatttgtaaa aattgattct tctagcgttg
      541 atatgaaaaa atataaattg cagaacaatg ttcgtcgttc tattaaatcc tcttcaatga
      601 actatgcgaa tgtcgctatt atgacagacg cagatcacga tggatagcct tcgggctatc
      661 tatagaaata cctcataatt aagagattat tggattaggt tgttagttta ctttaccaca
      721 aggatgtggt ataattaatt gggcaaaagc ttggcgtaat catggtcata gctgtttcct
      781 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      841 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      901 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      961 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1021 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1081 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1141 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1201 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1261 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1321 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1381 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1441 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1501 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1561 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     1621 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1681 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1741 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1801 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1861 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1921 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1981 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2041 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2101 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2161 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2221 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2281 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2341 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2401 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2461 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2521 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2581 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2641 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2701 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2761 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2821 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2881 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2941 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
