LOCUS       sequence-98534-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast beta tubulin (TUB2) - human beta4a 
            tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking 
            polyglutamate peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB2 - human TUBB4a Cterminal tail C127...
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(20..268)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     234..252
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     270..286
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     271..287
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             666..674
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gggcgaatgg gtaccggccg caaattaaag ccttcgagcg tcccaaaacc ttctcaagcc
       61 aggtttttca gtataatgtt acatgcgtac acgcgtctgt acagaaaaaa aagaaaaatt
      121 tgaaatataa ataacgttct taatactaac ataactataa aaaaataaat agggacctag
      181 acttcaggtt gtctaactcc ttccttttcg gttagagcgg atgtgggggg agggcgtgaa
      241 tgtaagcgtg acataactaa ttacatgact cgaggtcgac ggtatcgata agcttgatat
      301 cgacgtctta agcaacttct tcttcagctt cttcacagaa ttcaccttct tcagcagtag
      361 catcttggta ttgttggtat tcgctaacca gatcattcat attagattca gcctcagaga
      421 attccaattc gtccatacct tcactagtat accagtgcaa gaaagctttt cttttgaaca
      481 tagcggaaaa ttggtcacca actctcttga atagctcttg aatagatgtg gagttagcaa
      541 tgaaagtagc agccatgtcc aaaccttgag gagcgacaga acacacagca gtttgcacat
      601 tgttggggat ccattccacg aaatagtctg agtttttaga ttgcacttta tgcatttcat
      661 cttccacctc cttaacggaa actttacctc taaagaaggc tgcaacggta aggtatctac
      721 cgtttcttgg atcggcagca gccatcatgt tcttggcatc aaacatttgc tgtgttaatt
      781 cagggacagt caaagatcta aatgattgag agccaattgc cgtcaat
//
