LOCUS       sequence-98537-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast beta tubulin (TUB2) - human beta3 
            tubulin Cterminal tail (TUBB3) using a GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB2 - human TUBB3 Cterminal tail
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(20..267)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     233..251
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     269..285
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     270..286
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             684..692
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gggcgaatgg gtaccggccg caaattaaag ccttcgagcg gtcccaaaac cttctcaagc
       61 aaggtttcag tataatgtta catgcgtaca cgcgtctgta cagaaaaaaa agaaaaattt
      121 gaaatataaa taacgttctt aatactaaca taactataaa aaaataaata gggacctaga
      181 cttcaggttg tctaactcct tccttttcgg ttagagcgga tgtgggggga gggcgtgaat
      241 gtaagcgtga cataactaat tacatgactc gaggtcgacg gtatcgataa gcttgatatc
      301 gacgtcttta ttttggacct tgagcttcag attcttcttc atcatcttca tacatttcac
      361 cttcttcttc agcagtagca tcttggtatt gttggtattc gctaaccaga tcattcatat
      421 tagattcagc ctcagagaat tccaattcgt ccataccttc actagtatac cagtgcaaga
      481 aagcttttct tttgaacata gcggaaaatt ggtcaccaac tctcttgaat agctcttgaa
      541 tagatgtgga gttagcaatg aaagtagcag ccatgtccaa accttgagga gcgacagaac
      601 acacagcagt ttgcacattg ttggggatcc attccacgaa atagtctgag tttttagatt
      661 gcactttatg catttcatct tccacctcct taacgg
//
