LOCUS       sequence-98542-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of truncated form of human homodimeric kinesin-2 (KIF17) 
            amino acids 1-738.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pHR KIF17 (1-738)
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(97..120,121..156,157..180))
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             complement(211..372)
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     primer_bind     complement(434..454)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc aagcttgcat gcctgcaggt
       61 cgactctaga gtcgcggccg cttaactagt actgcctttt tcgaactgcg ggtggctcca
      121 ggcactgccg ccactgccgc cgccactgcc gccgcccttc tcaaattgtg gatgactcca
      181 gccgccgcca gagccgccag agccgccgcc ttcggttacc gtgaaggttt tggtagcgtc
      241 gtcgtaggtc cattcaccgt caacaccgtt gtcgttagcg tactgtttga aaactttttc
      301 cgcggtagca gcgtcaacag cttcggtggt ggtttcacct ttcagggttt taccgttcag
      361 gataagcttg tactcttcgg tccgacctcc acctttgtag agctcatcca tgccatgtgt
      421 aatcccagca gcagttacaa actcaagaag gaccatgtgg tcacgctttt cgttgggatc
      481 tttcgaaagg acagattgtg tcgacaggta atggttgtct ggtaaaagga cagggccatc
      541 gccaattgga gtattttgtt gataatggtc tgctagttga acggaaccat cttcaacgtt
      601 gtggcgaatt ttgaagttag ctttgattcc attcttttgt ttgtctgccg tgatgtatac
      661 attgtgtgag ttaaagttgt actcgagttt gtgtccaaga atgtttccat cttctttaaa
      721 atcaataccc tttaactcga tacgattaac aagggtatca ccttcaaact tgacttcagc
      781 acgcgtcttg taggtcccgt catctttgaa agatatagtg cgttcctgta cataaccttc
      841 gggcatggca ctcttg
//
