LOCUS       sequence-98547-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a 
            GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB1-Cterminal6xHis
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(20..267)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     233..251
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(277..294)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggcgattgg gtaccggccg caaattaaag ccttcgagcg tcccaaaacc ttctcaagca
       61 aggttttcag tataatgtta catgcgtaca cgcgtctgta cagaaaaaaa agaaaaattt
      121 gaaatataaa taacgttctt aatactaaca taactataaa aaaataaata gggacctaga
      181 cttcaggttg tctaactcct tccttttcgg ttagagcgga tgtgggggga gggcgtgaat
      241 gtaagcgtga cataactaat tacatgactc gagttagtgg tggtggtggt ggtgaaattc
      301 ctcttcctca gcgtatgagt cggcacccac ttcgatgtaa tctctttcta aagcagccaa
      361 atcttctcta gcttcggtga attcaccttc ttccatacct tcaccgacat accagtggac
      421 gaaagcacgt ttggcataca ttaaatcgaa ttttctatcg attctcttcc aagcctcagc
      481 aatggatgtg gtatttgaca acatacagac ggccctatcc acagtggcca attgtgagtt
      541 tggtgtggca gttggaggtt cgtagcaaat accgatcttg aaaccagttg gacaccaatc
      601 aaccaattgg acggtcttct tgtttttcac ctgctcgaca gctctttgaa catctcttgt
      661 taccacatca cccctgtata acagacaagt agccatgtat ttaccatctc ttggatcaca
      721 cttgaccatc tggttaccag gttcaaaaca agcgtttgta atttctgaca cagagttgga
      781 ctcatggaat gcctttgatt t
//
