LOCUS       sequence-98549-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a 
            GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB1-Cterminal6xHis
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..474
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    34..150
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     445..468
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     489..505
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     489..505
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tcactaaagg gaacaaaagc tggagctcta gtacggatta gaagccgccg agcgggtgaa
       61 gccctccgaa ggaagactct cctccgtgcg tcctcgtctt caccggtcgc gttcctgaaa
      121 cgcagatgtg cctcgcgccg cactgctccg aacaataaag attctacaat actagctttt
      181 atggttatga agaggaaaaa ttggcagtaa cctggcccca caaaccttca aatgaacgaa
      241 tcaaattaac aaccatagga tgataatgcg attagttttt tagccttatt tctggggtaa
      301 ttaatcagcg aagcgatgat ttttgatcta ttaacagata tataaatgca aaaactgcat
      361 aaccacttta actaatactt tcaacatttt cggtttgtat tacttcttat tcaaatgtaa
      421 taaaagtatc aacaaaaaat tgttaatata cctctatact ttaacgtcaa ggagaaaaaa
      481 ccccggattc tagaactagt ggatccaaaa tgagagaagt tattagtatt aatggtatgt
      541 tcgatttgcc cgtccaggct agatcttttt tttgttagtt cattttggcg tttcatcttt
      601 cattactaac ttcaacgcaa aatttttttt tttttgattt ctctttacag tcggtcaagc
      661 tggttgtcag attggtaatg cctgttggga attatattcc cttgagcacg gtattaagcc
      721 ggatggacat ctagaagatg gcctttcaaa gccgaaggga ggagaagagg gtttctccac
      781 g
//
