LOCUS       sequence-98553-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of yeast beta tubulin (TUB2) using a GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB2
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..466
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    25..142
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     437..460
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     481..497
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     481..497
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gggacaaaag ctggagctct agtacggatt agaagccgcc gagcgggtga cagccctccg
       61 aaggaagact ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg
      121 tgcctcgcgc cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat
      181 gaagaggaaa aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta
      241 acaaccatag gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag
      301 cgaagcgatg atttttgatc tattaacaga tatataaatg caaaaactgc ataaccactt
      361 taactaatac tttcaacatt ttcggtttgt attacttctt attcaaatgt aataaaagta
      421 tcaacaaaaa attgttaata tacctctata ctttaacgtc aaggagaaaa aaccccggat
      481 tctagaacta gtggatcccc gggaaaatga gagaaatcat tcatatctcg acaggtcagt
      541 gtggtaacca aattggtgct gcattctggg aaactatctg tggtgagcac ggtttggatt
      601 tcaatgggac atatcacggc catgacgata tccagaagga gagactgaac gtgtacttca
      661 acgaggcatc ttctgggaag tgggttccaa gatctattaa cgtcgatcta gaacctggga
      721 cgattgacgc agtacgcaat tctgccatcg ggaatttgtt tagacctgac aattatatct
      781 ttgggcaaag ttctgcgggc aacgtgt
//
