LOCUS       sequence-98561-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a 
            GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS426-TUB1-internal6xHis
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        16..456
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    16..132
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     427..450
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     471..487
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     471..487
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             737..754
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctggagctc tagtacggat tagaagccgc cgagcgggtg aagccctccg aaggaagact
       61 ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc
      121 cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa
      181 aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag
      241 gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg
      301 atttttgatc tattaacaga tatataaatg caaaaactgc ataaccactt taactaatac
      361 tttcaacatt ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa
      421 attgttaata tacctctata ctttaacgtc aaggagaaaa aaccccggat tctagaacta
      481 gtggatccaa aatgagagaa gttattagta ttaatggtat gttcgatttg cccgtccagg
      541 ctagatcttt tttttgttag ttcattttgg cgtttcatct ttcattacta acttcaacgc
      601 aaaatttttt tttttttgat ttctctttac agtcggtcaa gctggttgtc agattggtaa
      661 tgcctgttgg gaattatatt cccttgagca cggtattaag ccggatggac atctagaaga
      721 tggcctttca aagccgcatc atcaccatca tcacaa
//
