LOCUS       sequence-98562-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of yeast alpha tubulin (TUB1) - internal 6xHis without 
            Cterminal tail using a GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS426-TUB1-internal6xHis (1-440)
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(19..266)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     232..250
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gggcgatggg taccggccgc aaattaaagc cttcgagcgt cccaaaacct tctcaagcaa
       61 ggttttcagt ataatgttac atgcgtacac gcgtctgtac agaaaaaaaa gaaaaatttg
      121 aaatataaat aacgttctta atactaacat aactataaaa aaataaatag ggacctagac
      181 ttcaggttgt ctaactcctt ccttttcggt tagagcggat gtggggggag ggcgtgaatg
      241 taagcgtgac ataactaatt acatgactcg agttatgagt cggcacccac ttcgatgtaa
      301 tctctttcta aagcagccaa atcttctcta gcttcggtga attcaccttc ttccatacct
      361 tcaccgacat accagtggac gaaagcacgt ttggcataca ttaaatcgaa ttttctatcg
      421 attctcttcc aagcctcagc aatggatgtg gtatttgaca acatacagac ggccctatcc
      481 acagtggcca attgtgagtt tggtgtggca gttggaggtt cgtagcaaat accgatcttg
      541 aaaccagttg gacaccaatc aaccaattgg acggtcttct tgtttttcac ctgctcgaca
      601 gctctttgaa catctcttgt taccacatca cccctgtata acagacaagt agccatgtat
      661 ttaccatctc ttggatcaca cttgaccatc tggttaccag gttcaaaaca agcgtttgta
      721 atttctgaca cagagttgga ctcatggaat gcctttgatt tggataagac tggagaatat
      781 gaaact
//
