LOCUS       sequence-98563-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of yeast alpha tubulin (TUB1) - internal 6xHis without 
            Cterminal tail using a GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS426-TUB1-internal6xHis (1-440)
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        26..467
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    26..143
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     438..461
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     482..498
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     482..498
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             747..764
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggaacaaaa gctggagctc tagtacggat tagaagccgc cgagcgggtg acagccctcc
       61 gaaggaagac tctcctccgt gcgtcctcgt cttcaccggt cgcgttcctg aaacgcagat
      121 gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct tttatggtta
      181 tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaatgaac gaatcaaatt
      241 aacaaccata ggatgataat gcgattagtt ttttagcctt atttctgggg taattaatca
      301 gcgaagcgat gatttttgat ctattaacag atatataaat gcaaaaactg cataaccact
      361 ttaactaata ctttcaacat tttcggtttg tattacttct tattcaaatg taataaaagt
      421 atcaacaaaa aattgttaat atacctctat actttaacgt caaggagaaa aaaccccgga
      481 ttctagaact agtggatcca aaatgagaga agttattagt attaatggta tgttcgattt
      541 gcccgtccag gctagatctt ttttttgtta gttcattttg gcgtttcatc tttcattact
      601 aacttcaacg caaaattttt tttttttgat ttctctttac agtcggtcaa gctggttgtc
      661 agattggtaa tgcctgttgg gaattatatt cccttgagca cggtattaag ccggatggac
      721 atctagaaga tggcctttca aagccgcatc atcaccatca tcacaa
//
