LOCUS       sequence-98568-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) yeast codon-optimized yellow 
            fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1
            promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB789
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     96..114
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggacatttcg aagtactcag cgtaagtgat gtccacctcg atatgtgcat ctgtaaaagc
       61 aattgttcca ggaaccaggg cgtatctctt catagcctta tgcagttgct ctccagcggt
      121 tccatcttcc agcggataga atggcgccgg gcctttcttt atgtttttga cgtcttccat
      181 gttaattaag atgttaatat atcaacaaat aaagtgttgt tcgttggtgt ctctcttgcg
      241 ttgaaaagga tgaaaaggat gagatatgct tcaattatgg tagagtaatt ttccaacaat
      301 gaaatattag ctcctttata taatttttat tcaatggtac tgacttaaca aaggcaacaa
      361 gataaactct aaacttgtta ttctaaaacg tgagctgcag actttgttgg aggtttctat
      421 caaacacgaa actacctacg ccaacctatt ctttctacac tatatgaaaa aaatttccat
      481 atcgttttta cgcgtcctct attcaacacc caataagatt ctgcaaggat ttctgtgtcc
      541 tgaggatcaa aatgacgcgt ttacttagaa tttcaaaaaa agaagcaatt aaagtaacag
      601 aggatgaact tacccgacac tgtttacgcg ttttttcttt ttaaatttga tttgggtgcc
      661 atataatggg ataaaacgcg taaacaagct cgttccaggg ctacgaaata tttctaccca
      721 gaagtggtcg taaaataatt aacgatagta aacaaaggcc atagaaagtt cagcattgac
      781 ttggtaacga gattttcccc aatggaattc ctttgtatga aagattctcc tgaagagtaa
      841 ttctgccaat cagtacattt ctatggtaac gcctttgttt acgatatcca aatgcggcaa
      901 tccttctctg ttg
//
