LOCUS       sequence-98578-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Olenych SG, Claxton NS, Ottenberg GK, Davidson MW
  TITLE     The fluorescent protein color palette.
  JOURNAL   Curr Protoc Cell Biol. 2007 Sep;Chapter 21:Unit 21.5. doi: 
            10.1002/0471143030.cb2105s36.
  PUBMED    18228502
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Cell Biol."; date: "2007-09"; volume: "Chapter 21:Unit 21.5. doi"; 
            pages: " 10.1002/0471143030.cb2105s36"
COMMENT     Sequence Label: mCherry-Mito-7
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..98
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      126..135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             132..842
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(277..295)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     545..564
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcatgtccg tcctgacgcc gctgctgctg cggggcttga
       61 caggctcggc ccggcggctc ccagtgccgc gcgccaagat ccattcgttg ggggatccac
      121 cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
      181 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      241 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      301 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
      361 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
      421 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      481 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      541 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
      601 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
      661 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      721 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      781 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
      841 aaagcggccg cgactctaga tcataatcag
//
