LOCUS       sequence-98584-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yellow beetle luciferase with nuclear localization signal (NLS) and 
            ClpXP degron tag (ssrA), driven by LEU1 promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB756
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(98..116)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 aaaaagaaaa gttgccatgg gtgggggcag catggaagac gccaaaaaca taaagaaagg
       61 cccggcgcca ttctatcctc tagaggatgg aaccgctgga gagcaactgc ataaggctat
      121 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgaa
      181 catcacgtac gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
      241 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
      301 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
      361 tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa
      421 aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc agaaaattat
      481 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtatacgt tcgtcacatc
      541 tcatctacct cccggtttta atgaatacga ttttgtacca gagtcctttg atcgtgacaa
      601 aacaattgca ctgataatga attcctctgg atctactggg ttacctaagg gtgtggccct
      661 tccgcataga actgcctgcg tcagattctc gcatgccaga gatcctattt ttggcaatca
      721 aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt
      781 tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga
      841 a
//
