LOCUS       sequence-98586-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yellow beetle luciferase with nuclear localization signal (NLS) and 
            ClpXP degron tag (ssrA), driven by LEU1 promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB756
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..92)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(601..612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tctagaggat agaatggcgc cgggcctttc tttatgtttt tggcgtcttc catgctgccc
       61 ccacccatgg caacttttct tttttttttt ggtggagcca tggttaatta acccggggga
      121 tccactagtt ctagtttgga tggagtgtaa accaagattt aaaacagcaa ataataaaaa
      181 tcgatagcga caaaaactgt caatttcaat attctttata tttgttgact gcttagatat
      241 tttgagaaaa ttcagcggaa acagcgtgat gagtgagtta agttctgctg tttaaataag
      301 tattcaacta ctattgaagc cgactcatga agccggttac ggacaaaacc gggcaaattt
      361 cgccggtccc ggaattttcg tttccgcaat aaaagaaccg ctcatcatca tagcgccagg
      421 gtagtatact atagaaggtc agactaaact gagtcatcta gagtaatgac gccttagtag
      481 cttttacatc ttcataagaa aaggaaactt gtagaatggc ctggcgattt gtttgctttc
      541 ttgtgatgaa gaaatttcga tgcgattaac cggcaaaatc agtaaaggta tttcgcggag
      601 gcggccttca atcatcgaat actacgtctt aatatgatgt actgtggttc atattttcaa
      661 gtagtgttag taaatttgta tacgttcatg taagtgtgta tcttgagtgt ctgtatgggc
      721 gcataaacgt aagcgagact tccaaatgga gcaaacgaga agagatcttt aaagtattat
      781 agaagagctg ggcaggaact attatgacgt aaagccttga ccataataaa gacgattctt
      841 tgtccctcta tacaa
//
