LOCUS       sequence-98593-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of yeast beta tubulin (TUB2) - without Cterminal tail 
            using GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB2 (1-428)
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(18..265)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     231..249
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     267..283
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     268..284
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             726..734
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cgaattgggt accggccgca aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag
       61 gttttcagta taatgttaca tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga
      121 aatataaata acgttcttaa tactaacata actataaaaa aataaatagg gacctagact
      181 tcaggttgtc taactccttc cttttcggtt agagcggatg tggggggagg gcgtgaatgt
      241 aagcgtgaca taactaatta catgactcga ggtcgacggt atcgataagc ttgatatcga
      301 attcctgcag ttagtggtgg tggtggtgct attcaaaatt ctcagtgatt ggttcatctt
      361 ggttttgtgg agcaccaaaa tcgccatttt cgtcgacttc ttcatcatct tctacagtct
      421 actcttggta ttgttggtat tcgctaacca gatcattcat attagattca gcctcagaga
      481 attccaattc gtccatacct tcactagtat accagtgcaa gaaagctttt cttttgaaca
      541 tagcggaaaa ttggtcacca actctcttga atagctcttg aatagatgtg gagttagcaa
      601 tgaaagtagc agccatgtcc aaaccttgag gagcgacaga acacacagca gtttgcacat
      661 tgttggggat ccattccacg aaatagtctg agtttttaga ttgcacttta tgcatttcat
      721 cttccacctc cttaacggaa actttacctc taaagaaggc tgcaacggta aggtatctac
      781 cgtttcttgg atcggcagca gccatcatg
//
