LOCUS       sequence-98597-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) and yeast codon-optimized yellow 
            fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1
            promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB787
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
       61 aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt gggtggggga
      121 tcaatgtcta aaggtgaaga attattcact ggtgttgtcc caattttggt tgaattagat
      181 ggtgatgtta atggtcacaa attttctgtc tccggtgaag gtgaaggtga tgctacttac
      241 ggtaaattga ccttaaaatt gatttgtact actggtaaat tgccagttcc atggccaacc
      301 ttagtcacta ctttaggtta tggtttgcaa tgttttgcta gatacccaga tcatatgaaa
      361 caacatgact ttttcaagtc tgccatgcca gaaggttatg ttcaagaaag aactattttt
      421 ttcaaagatg acggtaacta caagaccaga gctgaagtca agtttgaagg tgatacctta
      481 gttaatagaa tcgaattaaa aggtattgat tttaaagaag atggtaacat tttaggtcac
      541 aaattggaat acaactataa ctctcacaat gtttacatca ctgctgacaa acaaaagaat
      601 ggtatcaaag ctaacttcaa aattagacac aacattgaag atggtggtgt tcaattagct
      661 gaccattatc aacaaaatac tccaattggt gatggtccag tcttgttacc agacaaccat
      721 tacttatcct atcaatctgc cttatccaaa gatccaaacg aaaagagaga ccacatggtc
      781 ttgttagaat ttgttactgc tgctggtatt acccatggta ttgatgaatc tcgagaagca
      841 tccaacttga acatttcgag aaagcttacc atatcaaccc catcatgctc tttcgaaaat
      901 tcaaatagca catccattcc ttcgcccgct tcctcatctc aaagccacac tccaatgaga
      961 aacatgagct cactctctga taacagcgtt ttcagccgga atatggaaca atcatcacca
     1021 atcactccaa gtatgtacca atttggtcag cagcagtcaa acagta
//
