LOCUS       sequence-98606-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis 
            with human alpha tubulin Cterminal tail (TUBA1a) using a GAL 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS426-TUB1-internal6xHis - human TUBA1a Cterm...
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        16..457
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    16..133
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     428..451
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     472..488
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     472..488
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             738..755
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctggagctc tagtacggat tagaagccgc cgagcgggtg acagccctcc gaaggaagac
       61 tctcctccgt gcgtcctcgt cttcaccggt cgcgttcctg aaacgcagat gtgcctcgcg
      121 ccgcactgct ccgaacaata aagattctac aatactagct tttatggtta tgaagaggaa
      181 aaattggcag taacctggcc ccacaaacct tcaaatgaac gaatcaaatt aacaaccata
      241 ggatgataat gcgattagtt ttttagcctt atttctgggg taattaatca gcgaagcgat
      301 gatttttgat ctattaacag atatataaat gcaaaaactg cataaccact ttaactaata
      361 ctttcaacat tttcggtttg tattacttct tattcaaatg taataaaagt atcaacaaaa
      421 aattgttaat atacctctat actttaacgt caaggagaaa aaaccccgga ttctagaact
      481 agtggatcca aaatgagaga agttattagt attaatggta tgttcgattt gcccgtccag
      541 gctagatctt ttttttgtta gttcattttg gcgtttcatc tttcattact aacttcaacg
      601 caaaattttt ttttttttga tttctcttta cagtcggtca agctggttgt cagattggta
      661 atgcctgttg ggaattatat tcccttgagc acggtattaa gccggatgga catctagaag
      721 atggcctttc aaagccgcat catcaccatc atcacaaggg aggagaagag gg
//
