LOCUS       sequence-98607-e        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green click beetle luciferase (Promega) driven by LEU1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB778
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             945..953
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 aagcgtgaga aaaatgtcat ctatggccct gagcctctcc atcctttgga ggatttgact
       61 gccggcgaaa tgctgtttcg tgctctccgc aagcactctc atttgcctca agccttggtc
      121 gatgtggtcg gcgatgaatc tttgagctac aaggagtttt ttgaggcaac cgtcttgctg
      181 gctcagtccc tccacaattg tggctacaag atgaacgacg tcgttagtat ctgtgctgaa
      241 aacaataccc gtttcttcat tccagtcatc gccgcatggt atatcggtat gatcgtggct
      301 ccagtcaacg agagctacat tcccgacgaa ctgtgtaaag tcatgggtat ctctaagcca
      361 cagattgtct tcaccactaa gaatattctg aacaaagtcc tggaagtcca aagccgcacc
      421 aactttatta agcgtatcat catcttggac actgtggaga atattcacgg ttgcgaatct
      481 ttgcctaatt tcatctctcg ctattcagac ggcaacatcg caaactttaa accactccac
      541 ttcgaccctg tggaacaagt tgcagccatt ctgtgtagca gcggtactac tggactccca
      601 aagggagtca tgcagaccca tcaaaacatt tgcgtgcgtc tgatccatgc tctcgatcca
      661 cgcgtgggca ctcagctgat tcctggtgtc accgtcttgg tctacttgcc tttcttccat
      721 gctttcggct ttagcattac tttgggttac tttatggtcg gtctccgcgt gattatgttc
      781 cgccgttttg atcaggaggc tttcttgaaa gccatccaag attatgaagt ccgcagtgtc
      841 atcaacgtgc ctagcgtgat cctgtttttg tctaagagcc cactcgtgga caagtacgac
      901 ttgtcttcac tgcgtgaatt gtgttgcggt gccgctccac tggctaagga ggtcgctgaa
      961 gtggccgcca aacgcttgaa tcttccaggg attcgttgtg gcttcggcct c
//
