LOCUS       sequence-98611-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast beta tubulin (TUB2) - human beta2 
            tubulin Cterminal tail (TUBB2a) using a GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB2 - human TUBB2a Cterminal tail
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(12..260)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     226..244
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     262..278
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     263..279
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             661..669
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gggtaccggc cgcaaattaa agccttcgag cgtcccaaaa ccttctcaag caaggttttt
       61 cagtataatg ttacatgcgt acacgcgtct gtacagaaaa aaaagaaaaa tttgaaatat
      121 aaataacgtt cttaatacta acataactat aaaaaaataa atagggacct agacttcagg
      181 ttgtctaact ccttcctttt cggttagagc ggatgtgggg ggagggcgtg aatgtaagcg
      241 tgacataact aattacatga ctcgaggtcg acggtatcga taagcttgat atcgacgtct
      301 taagcttcat cttcaccttc ttcttcttcg aattcacctt gttcatcagc agtagcctct
      361 tggtattgtt ggtattcgct aaccagatca ttcatattag attcagcctc agagaattcc
      421 aattcgtcca taccttcact agtataccag tgcaagaaag cttttctttt gaacatagcg
      481 gaaaattggt caccaactct cttgaatagc tcttgaatag atgtggagtt agcaatgaaa
      541 gtagcagcca tgtccaaacc ttgaggagcg acagaacaca cagcagtttg cacattgttg
      601 gggatccatt ccacgaaata gtctgagttt ttagattgca ctttatgcat ttcatcttcc
      661 acctccttaa cggaaacttt acctctaaag aaggctgcaa cggtaaggta tctaccgttt
      721 cttggatcgg cagcagccat catgttcttg gcatc
//
