LOCUS       sequence-98617-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast beta tubulin (TUB2) - human beta4a 
            tubulin Cterminal tail (TUBB4a) using a GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB2 - human TUBB4a Cterminal tail
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(14..261)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     227..245
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     263..279
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     264..280
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             659..667
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ttgggtaccg gccgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
       61 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
      121 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
      181 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
      241 gtgacataac taattacatg actcgaggtc gacggtatcg ataagcttga tatcgacgtc
      301 ttaagcaact tcttcttcag cttcttcttc gaattcacct tcttcagcag tagcatcttg
      361 gtattgttgg tattcgctaa ccagatcatt catattagat tcagcctcag agaattccaa
      421 ttcgtccata ccttcactag tataccagtg caagaaagct tttcttttga acatagcgga
      481 aaattggtca ccaactctct tgaatagctc ttgaatagat gtggagttag caatgaaagt
      541 agcagccatg tccaaacctt gaggagcgac agaacacaca gcagtttgca cattgttggg
      601 gatccattcc acgaaatagt ctgagttttt agattgcact ttatgcattt catcttccac
      661 ctccttaacg gaaactttac ctctaaagaa ggctgcaacg gtaaggtatc taccgtttct
      721 tggatcggca gcagccatca tgttcttggc atcaaacatt tgctgtgtta attca
//
