LOCUS       sequence-98625-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Amphioxus GFPa1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Bomati EK, Haley JE, Noel JP, Deheyn DD
  TITLE     Spectral and structural comparison between bright and dim green 
            fluorescent proteins in Amphioxus.
  JOURNAL   Sci Rep. 2014 Jun 27;4:5469. doi: 10.1038/srep05469.
  PUBMED    24968921
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-27"; volume: "4:5469. doi"; pages: " 
            10.1038/srep05469"
COMMENT     Sequence Label: bfloGFPa1
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(72..89)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttct aatggtgatg gtgatggtga gccagctcgt agaacgcctt ctccctctcg
      121 tccagggtga cttcggtctt ggtagccttc acgtggcact tgcggtaaac gaagaccggc
      181 tgcttcttca tgagatcggc tgaaaagggc ttgtcgaaga tgtagtggct cgacactcgg
      241 gccctgtagc gcttcccatt ctgcaggttg taactccagt cgaagctgtc cacgatggtg
      301 ttgttgttaa gatacgtctt cttggacacg cagccgtcct ggtcgacaat ctggctggtc
      361 atgactgggc cgtcgtcagg gaaaccgctt cccatcagct tcatgtcggc cttgatatgg
      421 gaaccctcgt aggagtactt aaactcggta gacagcttgc ctccgtcttc aaagtcgaac
      481 acgcggtaga ctgcataccc cgatccttcc aacatggaga cctggaaagg cgagggtccg
      541 tccgggtagg ggaggtactg gtagtacccg tacccgaggt gggggatcat caagtaggga
      601 gagaacttca gggcaccctt ggtggatttc gctgtggtca ccagcgagcc ggcgttcggg
      661 tcgccttttc ctccccccac catgtcgaac tcgtggccgt tgatggagcc gtgaaggtgg
      721 atgtcgtggg ttgcgggcag aggcatatga tggct
//
