LOCUS       sequence-98627-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression of SspB Nano.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pQE-80L MBP-SspB Nano
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..33
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             37..366
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     terminator      394..488
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(598..617)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gagggaacga ccgaaaacct gtattttcag ggatccagct ccccgaaacg ccctaagctg
       61 ctgcgtgaat attacgattg gctggttgat aacagcttta ccccatatct ggtggtggat
      121 gccacatacc tgggcgtgaa cgtgcccgtg gagtatgtga aagacggtca gatcgtgctg
      181 aatctgtctg caagtgcgac cggcaacctg caactgacaa atgattttat ccagttcaac
      241 gcccgcttta agggcgtgtc tcgtgaactg tatatcccga tgggtgccgc tctggccatt
      301 tacgctcgcg agaacggcga tggtgtgatg ttcgaaccag aagaaatcta tgacgagctg
      361 aatattggtt aaaagcttaa ttagctgagc ttggactcct gttgatagat ccagtaatga
      421 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
      481 gtgagaatcc aagctagctt ggcgagattt tcaggagcta aggaagctaa aatggagaaa
      541 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
      601 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
      661 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
      721 gcccgcctga tgaatgctca tccggaattt cgtatg
//
