LOCUS       sequence-98628-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Amphioxus GFPa1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Bomati EK, Haley JE, Noel JP, Deheyn DD
  TITLE     Spectral and structural comparison between bright and dim green 
            fluorescent proteins in Amphioxus.
  JOURNAL   Sci Rep. 2014 Jun 27;4:5469. doi: 10.1038/srep05469.
  PUBMED    24968921
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-27"; volume: "4:5469. doi"; pages: " 
            10.1038/srep05469"
COMMENT     Sequence Label: bfloGFPa1
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             752..769
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             794..811
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tcatatgcct ctgcccgcaa cccacgacat
      121 ccaccttcac ggctccatca acggccacga gttcgacatg gtggggggag gaaaaggcga
      181 cccgaacgcc ggctcgctgg tgaccacagc gaaatccacc aagggtgccc tgaagttctc
      241 tccctacttg atgatccccc acctcgggta cgggtactac cagtacctcc cctacccgga
      301 cggaccctcg cctttccagg tctccatgtt ggaaggatcg gggtatgcag tctaccgcgt
      361 gttcgacttt gaagacggag gcaagctgtc taccgagttt aagtactcct acgagggttc
      421 ccatatcaag gccgacatga agctgatggg aagcggtttc cctgacgacg gcccagtcat
      481 gaccagccag attgtcgacc aggacggctg cgtgtccaag aagacgtatc ttaacaacaa
      541 caccatcgtg gacagcttcg actggagtta caacctgcag aatgggaagc gctacagggc
      601 ccgagtgtcg agccactaca tcttcgacaa gcccttttca gccgatctca tgaagaagca
      661 gccggtcttc gtttaccgca agtgccacgt gaaggctacc aagaccgaag tcaccctgga
      721 cgagagggag aaggcgttct acgagctggc tcaccatcac catcaccatt agaagcttgc
      781 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      841 ggaagctgag ttggctgctg
//
