LOCUS       sequence-98638-e         238 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Fluorescence Lifetime Imaging Microscopy 
            (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent 
            manner in an AAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Chen Y, Saulnier JL, Yellen G, Sabatini BL
  TITLE     A PKA activity sensor for quantitative analysis of endogenous GPCR 
            signaling via 2-photon FRET-FLIM imaging.
  JOURNAL   Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. 
            eCollection 2014.
  PUBMED    24765076
REFERENCE   2  (bases 1 to 238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Pharmacol."; date: "2014-04-2"; volume: "5:56. doi"; pages: " 
            10.3389/fphar.2014.00056. eCollection 2014"
COMMENT     Sequence Label: AAV-FLEX-FLIM-AKAR
FEATURES             Location/Qualifiers
     source          1..238
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..38
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    101..134
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccccataact tcgtataaag tatcctatac gaagttatat caaaatagga agaccaatgc
       61 ttcaccatcg acccgaattg ccaagcatca ccatcgaccc ataacttcgt ataatgtatg
      121 ctatacgaag ttatactagg gatcccctta ctcgatgttg tgcctgattt tgaagttgac
      181 cttgataccg tttttctgct tgtcggccat aatatagacg ttcacgctat tccagttg
//
