LOCUS       sequence-98640-e         270 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR
            in a Cre dependent manner in an AAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Chen Y, Saulnier JL, Yellen G, Sabatini BL
  TITLE     A PKA activity sensor for quantitative analysis of endogenous GPCR 
            signaling via 2-photon FRET-FLIM imaging.
  JOURNAL   Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. 
            eCollection 2014.
  PUBMED    24765076
REFERENCE   2  (bases 1 to 270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Pharmacol."; date: "2014-04-2"; volume: "5:56. doi"; pages: " 
            10.3389/fphar.2014.00056. eCollection 2014"
COMMENT     Sequence Label: AAV-FLEX-FLIM-AKART391A
FEATURES             Location/Qualifiers
     source          1..270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..42
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    105..138
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggatccccat aacttcgtat aaagtatcct atacgaagtt atatcaaaat aggaagacca
       61 atgcttcacc atcgacccga attgccaagc atcaccatcg acccataact tcgtataatg
      121 tatgctatac gaagttatac tagggatccc cttactcgat gttgtgcctg attttgaagt
      181 tgaccttgat accgtttttc tgcttgtcgg ccataatata gacgttcacg ctattccagt
      241 tgtactccag cttatgcccg aggatatttc
//
