LOCUS       sequence-98643-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast beta tubulin (TUB2) - human beta7 
            tubulin Cterminal tail (TUBB7) using a GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB2 - human TUBB7 Cterminal tail
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..268)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     234..252
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     270..286
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     271..287
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             639..647
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gggcgaattg ggtaccggcc gcaaattaaa gccttcgagc gtcccaaaac cttctcaagc
       61 aaggttttca gtataatgtt acatgcgtac acgcgtctgt acagaaaaaa aagaaaaatt
      121 tgaaatataa ataacgttct taatactaac ataactataa aaaaataaat agggacctag
      181 acttcaggtt gtctaactcc ttccttttcg gttagagcgg atgtgggggg agggcgtgaa
      241 tgtaagcgtg acataactaa ttacatgact cgaggtcgac ggtatcgata agcttgatat
      301 cgacgtctta aacaccttca ccttcagcag tagcatcttg gtattgttgg tattcgctaa
      361 ccagatcatt catattagat tcagcctcag agaattccaa ttcgtccata ccttcactag
      421 tataccagtg caagaaagct tttcttttga acatagcgga aaattggtca ccaactctct
      481 tgaatagctc ttgaatagat gtggagttag caatgaaagt agcagccatg tccaaacctt
      541 gaggagcgac agaacacaca gcagtttgca cattgttggg gatccattcc acgaaatagt
      601 ctgagttttt agattgcact ttatgcattt catcttccac ctccttaacg gaaactttac
      661 ctctaaagaa ggctgcaacg gtaaggtatc taccgtttct tggatcggca gcagccatca
      721 tgttcttggc atcaaacatt tgctgtgtta attcagggac agtcaaagat ctaaatgatt
      781 gagagccaa
//
