LOCUS       sequence-98650-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression of SspB Micro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pQE-80L MBP-SspB Micro
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     475..498
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             565..585
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(589..795,796..798,799..918)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
ORIGIN
        1 aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa
       61 ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa
      121 gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt
      181 ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc
      241 ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg
      301 ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc
      361 actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc
      421 tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa
      481 gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac
      541 aacctcggga tcgagggaac gaccgaaaac ctgtattttc agggatccag ctccccgaaa
      601 cgccctaagc tgctgcgtga atattacgat tggctggttg ataacagctt taccccatat
      661 ctggtggtgg atgccacata cctgggcgtg aacgtgcccg tggagtatgt gaaagacggt
      721 cagatcgtgc tgaatctgtc tgcaagtgcg accggcaacc tgcaactgac aaatgatttt
      781 atccagttca acgcccagtt taagggcgtg tctcgtgaac tgtatatccc gatgggtgcc
      841 gctctggcca tttacgctcg cgagaacggc gatggtgtga tgttcgaacc agaagaaatc
      901 tatgacgagc tgaatattgg ttaaaagctt
//
