LOCUS       sequence-98665-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Zhang Z, Xu J, Koppetsch BS, Wang J, Tipping C, Ma S, Weng Z, 
            Theurkauf WE, Zamore PD
  TITLE     Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 
            ligase and Tudor domains.
  JOURNAL   Mol Cell. 2011 Nov 18;44(4):572-84. doi: 
            10.1016/j.molcel.2011.10.011.
  PUBMED    22099305
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.10"; journalName: "Mol Cell"; date: 
            "2011-11-18- 18"; volume: "44"; issue: "4"; pages: "572-84"
COMMENT     Sequence Label: pUASp-Flag-Myc-Qin-E3-attB
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      297..306
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             306..371
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      387..396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             396..425
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             435..464
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             474..503
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             513..542
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             552..581
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             615..644
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    657..681
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttaattcagt gcacgtttgc ttgttgagag gaaaggttgt gtgcggacga attttttttt
       61 gaaaaccggt gatagagcct gaaccagaaa agataaaaga aggctatacc agtgggagta
      121 cacaaacaga gtaagtttga atagtaaaaa aaatcattta tgtaaacaat aacgtgactg
      181 tgcgttaggt cctgttcatt gatcgaggcc tgtctagaga agcttgttcg aatctcgagt
      241 gcgcgcttcc ggaggtatac acctaggcgg taccactgca gtgaattcgg agctccgcca
      301 ccatggacta caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg
      361 acgatgacaa gcaccggttg agctccgcca ccatggagca aaagctcatt tctgaagagg
      421 acttgaatga aatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagcaaa
      481 agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct gaagaggact
      541 tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg gagagcttgg
      601 gcgacctcac catggagcaa aagctcattt ctgaagagga cttgaatcac cggtatacaa
      661 gtttgtacaa aaaagcaggc ttcatgattg ccgacgacag tggaataaag ccctttgatc
      721 ccaagtttgt ggcgtccaat ctgcagaatc ttctgcggtg cccaaaa
//
