LOCUS       sequence-98668-e         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Zhang Z, Xu J, Koppetsch BS, Wang J, Tipping C, Ma S, Weng Z, 
            Theurkauf WE, Zamore PD
  TITLE     Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 
            ligase and Tudor domains.
  JOURNAL   Mol Cell. 2011 Nov 18;44(4):572-84. doi: 
            10.1016/j.molcel.2011.10.011.
  PUBMED    22099305
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.10"; journalName: "Mol Cell"; date: 
            "2011-11-18- 18"; volume: "44"; issue: "4"; pages: "572-84"
COMMENT     Sequence Label: pUASp-Flag-Myc-Qin-Tudor-attB
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      298..307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             307..372
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      388..397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             397..426
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             436..465
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             475..504
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             514..543
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             553..582
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ttaattcagt gcacgtttgc ttgttgagag gaaaggttgt gtgcggacga attttttttt
       61 gaaaaccggt gatagagcct gaaccagaaa agataaaaga aggctatacc agtgggagta
      121 cacaaacaga gctaagtttg aatagtaaaa aaaatcattt atgtaaacaa taacgtgact
      181 gtgcgttagg tcctgttcat tgatcgaggc ctgtctagag aagcttgttc gaatctcgag
      241 tgcgcgcttc cggaggtata cacctaggcg gtaccactgc agtgaattcg gagctccgcc
      301 accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
      361 gacgatgaca agcaccggtt gagctccgcc accatggagc aaaagctcat ttctgaagag
      421 gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa
      481 aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac
      541 ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagagcttg
      601 ggcgacctca ccatggagca aaagctcatt tc
//
