LOCUS       sequence-98675-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast beta tubulin (TUB2) - human beta8 
            tubulin Cterminal tail (TUBB8) using a GAL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB2 - human TUBB8 Cterminal tail
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..269)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     235..253
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     271..287
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     272..288
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             667..675
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gggcgaattg ggtaccggcc gcaaattaaa gccttcgagc gtcccaaaac cttctcaagc
       61 aaggttttca gtataatgtt acatgcgtac acgcgctctg tacagaaaaa aaagaaaaat
      121 ttgaaatata aataacgttc ttaatactaa cataactata aaaaaataaa tagggaccta
      181 gacttcaggt tgtctaactc cttccttttc ggttagagcg gatgtggggg gagggcgtga
      241 atgtaagcgt gacataacta attacatgac tcgaggtcga cggtatcgat aagcttgata
      301 tcgacgtctt aagcaacttc ttcttcagca tattcttcat cttcttcttc ttcagcagta
      361 gcatcttggt attgttggta ttcgctaacc agatcattca tattagattc agcctcagag
      421 aattccaatt cgtccatacc ttcactagta taccagtgca agaaagcttt tcttttgaac
      481 atagcggaaa attggtcacc aactctcttg aatagctctt gaatagatgt ggagttagca
      541 atgaaagtag cagccatgtc caaaccttga ggagcgacag aacacacagc agtttgcaca
      601 ttgttgggga tccattccac gaaatagtct gagtttttag attgcacttt atgcatttca
      661 tcttccacct ccttaacgga aactttacct ctaaagaagg ctgcaacggt aaggtatcta
      721 ccgtttcttg gatcgg
//
