LOCUS       sequence-98678-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast beta tubulin (TUB2) - human beta2 
            tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking 
            polyglutamate peptide .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB2 - human TUBB2a Cterminal tail C127...
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(12..259)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     225..243
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     261..277
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     262..278
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             660..668
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gggtaccggc cgcaaattaa agccttcgag cgtcccaaaa ccttctcaag caaggttttc
       61 agtataatgt tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata
      121 aataacgttc ttaatactaa cataactata aaaaaataaa tagggaccta gacttcaggt
      181 tgtctaactc cttccttttc ggttagagcg gatgtggggg gagggcgtga atgtaagcgt
      241 gacataacta attacatgac tcgaggtcga cggtatcgat aagcttgata tcgacgtctt
      301 aagcttcatc ttcaccttct tcttcttcga aacaaccttg ttcatcagca gtagcatctt
      361 ggtattgttg gtattcgcta accagatcat tcatattaga ttcagcctca gagaattcca
      421 attcgtccat accttcacta gtataccagt gcaagaaagc ttttcttttg aacatagcgg
      481 aaaattggtc accaactctc ttgaatagct cttgaataga tgtggagtta gcaatgaaag
      541 tagcagccat gtccaaacct tgaggagcga cagaacacac agcagtttgc acattgttgg
      601 ggatccattc cacgaaatag tctgagtttt tagattgcac tttatgcatt tcatcttcca
      661 cctccttaac ggaaacttta cctctaaaga aggctgcaac ggtaaggtat ctaccgtttc
      721 ttggatcggc agcagccatc atgttcttgg catcaaacat ttgctgtgtt taattcaggg
      781 acagtcaaag atctaaatga ttgagag
//
