LOCUS       sequence-98681-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis 
            with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for 
            crosslinking polyglutamate peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS426-TUB1-internal6xHis - human TUBA1a Cterm...
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(12..259)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     225..243
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gggtaccggc cgcaaattaa agccttcgag cgtcccaaaa ccttctcaag caaggttttc
       61 agtataatgt tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata
      121 aataacgttc ttaatactaa cataactata aaaaaataaa tagggaccta gacttcaggt
      181 tgtctaactc cttccttttc ggttagagcg gatgtggggg gagggcgtga atgtaagcgt
      241 gacataacta attacatgac tcgagttaat attcttcacc ttcttcacaa ccttcacctt
      301 caactgagtc ggcacccact tcgatgtaat ctctttctaa agcagccaaa tcttctctag
      361 cttcggtgaa ttcaccttct tccatacctt caccgacata ccagtggacg aaagcacgtt
      421 tggcatacat taaatcgaat tttctatcga ttctcttcca agcctcagca atggatgtgg
      481 tatttgacaa catacagacg gccctatcca cagtggccaa ttgtgagttt ggtgtggcag
      541 ttggaggttc gtagcaaata ccgatcttga aaccagttgg acaccaatca accaattgga
      601 cggtcttctt gtttttcacc tgctcgacag ctctttgaac atctcttgtt accacatcac
      661 ccctgtataa cagacaagta gccatgtatt taccatctct tggatcacac ttgaccatct
      721 ggttaccagg gttcaaaaca agcgtttgta atttctgaca cagag
//
