LOCUS       sequence-98682-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis 
            with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for 
            crosslinking polyglutamate peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS426-TUB1-internal6xHis - human TUBA1a Cterm...
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        29..470
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    29..146
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     441..464
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     485..501
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     485..501
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             751..768
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taagggaaca aaagctggag ctctagtacg gattagaagc cgccgagcgg gtgacagccc
       61 tccgaaggaa gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc ctgaaacgca
      121 gatgtgcctc gcgccgcact gctccgaaca ataaagattc tacaatacta gcttttatgg
      181 ttatgaagag gaaaaattgg cagtaacctg gccccacaaa ccttcaaatg aacgaatcaa
      241 attaacaacc ataggatgat aatgcgatta gttttttagc cttatttctg gggtaattaa
      301 tcagcgaagc gatgattttt gatctattaa cagatatata aatgcaaaaa ctgcataacc
      361 actttaacta atactttcaa cattttcggt ttgtattact tcttattcaa atgtaataaa
      421 agtatcaaca aaaaattgtt aatatacctc tatactttaa cgtcaaggag aaaaaacccc
      481 ggattctaga actagtggat ccaaaatgag agaagttatt agtattaatg gtatgttcga
      541 tttgcccgtc caggctagat cttttttttg ttagttcatt ttggcgtttc atctttcatt
      601 actaacttca acgcaaaatt tttttttttt tgatttctct ttacagtcgg tcaagctggt
      661 tgtcagattg gtaatgcctg ttgggaatta tattcccttg agcacggtat taagccggat
      721 ggacatctag aagatggcct ttcaaagccg catcatcacc atcatcacaa gggaggagaa
      781 gagggtttct ccacg
//
