LOCUS       sequence-98689-e        1470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  green ddFP based caspase-3 biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: GA-DEVD-GB-NES
FEATURES             Location/Qualifiers
     source          1..1470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagcttgcca ccatggcgag caagagcgag gaggtcatca aagagttcat gcgcttcaag
       61 gtgcgcttgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccttacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccccctgatc atgtacggct ccaagatgta cgtgaagcac
      241 cccgccgacg tccccgatta catgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgcact tcgaggacgg cggtctggtg accgcgactc aggactcctc cctgcaggac
      361 ggcacgctga tctacaaggt gaagattcgc ggcaccaact tcccccccga cggccccgta
      421 atgcagaaga agaccttggg ctgggattat gccaccgagc gcctgtaccc caaggacggc
      481 gtgctgaagg gcgagcttct ggggcgcctg aagctgaagg acggcggcct caacctggtg
      541 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctacttcgtg
      601 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
      661 cgctccgagg gccgccacca cctggggcat ggtacaggta gcacaggcag cggtagctcc
      721 ggtaccgcct ccggcgatga ggtggatgga gccaccatca aagagttcat gcgcttcaag
      781 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
      841 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
      901 tttgcctggg acatcctgtc cccccagatc atgtacggct ccaaagcgta cgtgaggcac
      961 cccgccgaca tccccgatta caagaagctg cccttccccg agggcttcaa gtgggagcgc
     1021 gtgatgaact tcgaagacgg cggtctggtg accgttaccc aggactcctc cctgcaggac
     1081 ggcacgctga tctgcaaggt gaagattcgc ggcaccaact tcccccccga cggccccgta
     1141 atgcagaaga agaccatggg ctgggaggcc tccaccgaga tgctgtaccc caaggacggc
     1201 gtgctgaagg gccatagcta tcaggccctg aagctgaagg acggcggcca ctacctggtg
     1261 gagttcgaga ccatctacat ggccaagaag cccgtgcaac tgcccggcga ttactgtgtg
     1321 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacggg
     1381 cgctccgagg gccgccaccg tctgggcatg gacgagcggt acaagttagc cttgaaatta
     1441 gcaggtcttg atatcgggag ctaactcgag
//
