LOCUS       sequence-98700-e        4526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry tagged fusion proteins in worms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4526)
  TITLE     Johnson lab C. elegans plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHT101-mCherry
FEATURES             Location/Qualifiers
     source          1..4526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             146..166
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1966..1988)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2088..2107
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2185..2203)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2271..2375
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2376..2444,2445..3236)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2594..2613)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3407..3995
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3896..3915
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4149..4166
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4283..4304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4319..4324,4325..4342,4343..4349)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4357..4373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4362..4384
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4381..4397
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4381..4397
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggagggt
      121 accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg taggcttaat
      181 ggtctcaaag ggtgaagaag ataacatggc aattattaaa gagtttatgc gtttcaaggt
      241 gcatatggag ggatctgtca atgggcatga gtttgaaatt gaaggtgaag gagaaggccg
      301 accatatgag ggaacacaaa ccgcaaaact aaaggtaagt ttaaacatat atatactaac
      361 taaccctgat tatttaaatt ttcaggtaac taaaggcgga ccattaccat tcgcctggga
      421 catcctctct ccacagttca tgtatggaag taaagcttat gttaaacatc cggcagatat
      481 accagattat ttgaaacttt cattcccgga gggttttaag tgggaacgcg taatgaattt
      541 tgaagacgga ggagttgtta cagtgacgca agactcaagg taagtttaaa cagttcggta
      601 ctaactaacc atacatattt aaattttcag cctccaagat ggagaattta tttataaagt
      661 caaacttcga ggaacgaatt tcccctcgga tggacctgtt atgcagaaga agactatggg
      721 atgggaagct tcaagtgaaa gaatgtaccc tgaagacggt gctcttaagg gagagattaa
      781 acaacgtctt aaattgaaag atggaggaca ttacgatgct gaggtaagtt taaacatgat
      841 tttactaact aactaatctg atttaaattt tcaggtgaag acaacttaca aagccaaaaa
      901 accagttcag ctgccaggag cgtacaatgt taatattaaa ctggatatca cctcccacaa
      961 cgaggattac actatcgttg agcaatatga aagagctgaa gggcggcact cgacaggtgg
     1021 catggatgaa ttgtataaga tctagccatg gaattccaac tgagcgccgg tcgctaccat
     1081 taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta agtttaaact
     1141 gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc gtgcctctga
     1201 cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc
     1261 cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa
     1321 gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt
     1381 cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat
     1441 gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa
     1501 aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta
     1561 tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg
     1621 gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg
     1681 ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa
     1741 atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct
     1801 cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg
     1861 ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta ctagtcggcc
     1921 gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     1981 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     2041 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     2101 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2161 ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca
     2221 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2281 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2341 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2401 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2461 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2521 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2581 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2641 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2701 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2761 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2821 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2881 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2941 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3001 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3061 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3121 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3181 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3241 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3301 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3361 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3421 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3481 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3541 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3601 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3661 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3721 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3781 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3841 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3901 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3961 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4021 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4081 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4141 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     4201 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4261 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4321 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4381 caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac
     4441 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
     4501 atacgctaac aacttggaaa tgaaat
//
