LOCUS       sequence-9871-ex        4002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM1.26
FEATURES             Location/Qualifiers
     source          1..4002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1210..1238)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1986..2006
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    2048..2171
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2244..2262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2267..2283)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2267..2283)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2385..2404)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2396..3205
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2463..2482)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3352..3940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3841..3860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgct gcaggtcgac tctagaggat caagagcatt
      721 tgaatcagaa tatggagaac ggagcatgag cattttcgaa gttttttaga tgcactagaa
      781 caaagcgtgt tggcttcctc tgagcccgct ttccttatat acccgcattc tgcagcctta
      841 cagaatgttc tagaaggtcc tagatgcatt cgtttgaaaa tactcccggt gggtgcaaag
      901 agacgcagac ggaaaatgta tctgggtctc tttattgtgt acactacttt tccatgtacc
      961 gaatgtgagt cgccctcctt ttgcaacaag cagctcgaat gttctagaaa aaggtggaaa
     1021 atagtataaa taccgttgaa aataaatacc gaacaacatt tgctctaatt gtgaaattag
     1081 aaatcttcaa actataatca tctcactgga tcctatcgat tcgcggccgc tgtacacccg
     1141 ggtgccctat agtgagtcgt attggtaccg gtagaaaaaa tgagtaaagg agaagaactt
     1201 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     1261 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     1321 tgcactactg gaaaactacc tgttccatgg gtaagtttaa acatatatat actaactaac
     1381 cctgattatt taaattttca gccaacactt gtcactactt tctgttatgg tgttcaatgc
     1441 ttctcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
     1501 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtaag
     1561 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtgc tgaagtcaag
     1621 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     1681 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     1741 gcagacaaac aaaagaatgg aatcaaagtt gtaagtttaa acatgatttt actaactaac
     1801 taatctgatt taaattttca gaacttcaaa attagacaca acattgaaga tggaagcgtt
     1861 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1921 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1981 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggacgaacta
     2041 tacaaagcaa gtttgtacaa aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat
     2101 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatg
     2161 cagtcactat gaatcaacta cttagatggt attagtgacc tgtagaattc gagctctaga
     2221 gctgcagggc ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2281 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2341 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     2401 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     2461 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     2521 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     2581 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     2641 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     2701 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     2761 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     2821 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     2881 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     2941 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     3001 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     3061 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     3121 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     3181 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     3241 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     3301 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3361 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3421 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3481 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3541 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3601 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3661 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3721 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     3781 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3841 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3901 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3961 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
