LOCUS       sequence-98712-e         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MethyloBrick cloning vector, no promoter; TcR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Schada von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt 
            JA, Erb TJ
  TITLE     A Set of Versatile Brick Vectors and Promoters for the Assembly, 
            Expression, and Integration of Synthetic Operons in Methylobacterium
            extorquens AM1 and Other Alphaproteobacteria.
  JOURNAL   ACS Synth Biol. 2014 Aug 8.
  PUBMED    25105793
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Aug 8."
COMMENT     Sequence Label: pTE100
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(17..35)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
       61 atggtttctt agcacccttt ctcggtcctt caacgttcct gacaacgagc ctccttttcg
      121 ccaatccatc gacaatcacc gcgagtccct gctcgaacgc tgcgtccgga ccggcttcgt
      181 cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg agcctgttca acggtgccgc
      241 cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag cacggcccca acagtgaagt
      301 agctgattgt catcagcgca ttgacggcgt ccccggccga aaaacccgcc tcgcagagga
      361 agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc cggtcgcgtg ccggcatgga
      421 tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa gctgcgggca ttcccgatca
      481 gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg cgtatgattc tccgccagca
      541 tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct gaagtgccag taaagcgccg
      601 gctgctgaa
//
