LOCUS       sequence-98714-e         449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MethyloBrick cloning vector, no promoter; TcR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Schada von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt 
            JA, Erb TJ
  TITLE     A Set of Versatile Brick Vectors and Promoters for the Assembly, 
            Expression, and Integration of Synthetic Operons in Methylobacterium
            extorquens AM1 and Other Alphaproteobacteria.
  JOURNAL   ACS Synth Biol. 2014 Aug 8.
  PUBMED    25105793
REFERENCE   2  (bases 1 to 449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Aug 8."
COMMENT     Sequence Label: pTE100
FEATURES             Location/Qualifiers
     source          1..449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(87..104)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(87..103)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(96..118)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(312..331)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgtacatct agaaataaga aggagatata attaatccat ggcaattgaa gcttagatct
       61 tgactagtcc tgcaggtacc taattcactg gccgtcgttt tacaacgtcg tgactgggaa
      121 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      181 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      241 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      301 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
      361 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
      421 gtgaccgtct ccgggagctg catgtgtca
//
