LOCUS       sequence-98715-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to make mCherry-Parkin stable cell line with retrovirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Yamano K, Fogel AI, Wang C, van der Bliek AM, Youle RJ
  TITLE     Mitochondrial Rab GAPs govern autophagosome biogenesis during 
            mitophagy.
  JOURNAL   Elife (Cambridge). 2014 Feb 25;3:e01612. doi: 10.7554/eLife.01612.
  PUBMED    24569479
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife 
            (Cambridge)."; date: "2014-02-25"; volume: "3:e01612. doi"; pages: "
            10.7554/eLife.01612"
COMMENT     Sequence Label: pBMN-mCherry-Parkin
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    32..406
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     344..360
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      415..424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(566..584)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cctcactcct tctctaggcg cccccatatg gccatatgag atcttatatg gggcaccccc
       61 gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc cctctctcca
      121 agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc tggcggcagc
      181 ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg gcgacacagt
      241 gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac cttacacagt
      301 cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga tacacgccgc
      361 ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgccgg atccgccacc
      421 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
      481 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      541 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      601 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      661 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      721 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      781 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      841 atg
//
