LOCUS       sequence-98716-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MethyloBrick expression vector, mxaF promoter; TcR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Schada von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt 
            JA, Erb TJ
  TITLE     A Set of Versatile Brick Vectors and Promoters for the Assembly, 
            Expression, and Integration of Synthetic Operons in Methylobacterium
            extorquens AM1 and Other Alphaproteobacteria.
  JOURNAL   ACS Synth Biol. 2014 Aug 8.
  PUBMED    25105793
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Aug 8."
COMMENT     Sequence Label: pTE102
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(16..32)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(383..399)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(396..418)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    407..423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(431..437,438..455,456..461))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    476..497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttggcgtaa tcatggtcat agctgtttcc tgtgtgagat ccgcggtatc tctcagacgt
       61 ttttctgagg ttccaggctc gctcggaagt catccgaagt gccgcatcgt gaatctaagg
      121 atatttttag atttgcgcgg tcttatgaaa gacttattag cgtcccttcg cctatatttt
      181 ctaggctttg attggacgcg atgtctttag ctggtcggac atttcttatc aggacgataa
      241 ggcaattcta aaagtgccaa ggcgtagtgt cgcaacgtat tggcatcgtc ttgaccgggg
      301 ccgggaccca tcgcaccgtt gtcgtcaacg ggccctcgcg aagcggcccg accaagcggg
      361 aagctagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      421 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      481 tgagctaact cacattaatt atatctcctt cttatttcta gatgtacaga attccatgtg
      541 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      601 taggctccgc ccccctgac
//
