LOCUS       sequence-9872-ex        4010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM1.27
FEATURES             Location/Qualifiers
     source          1..4010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1218..1246)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1994..2014
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    2056..2179
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2252..2270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2275..2291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2275..2291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2393..2412)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2404..3213
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2471..2490)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3360..3948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3849..3868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgct gcaggtcgac tctagaggat caccaaaaac
      721 ggaacgttga gctggacgga aatagtggta aagtgacatg attatagttt gaagatttct
      781 aatttcacaa ttagagcaaa tgttgttcgg tatttatttt caacggtatt tatactattt
      841 tccacctttt tctagaacat tcgagctgct tgttgcaaaa ggagggcgac tcacattcgg
      901 tacatggaaa agtagtgtac acaataaaga gacccagata cattttccgt ctgcgtctct
      961 ttgcacccac cgggagtatt ttcaaacgaa tgcatctagg accttctaga acattctgta
     1021 aggctgcaga atgcgggtat ataaggaaag cgggctcaga ggaagccaac acgctttgtt
     1081 ctagtgcatc taaaaaactt cgaaaatcct catcgggatc ctatcgattc gcggccgctg
     1141 tacacccggg tgccctatag tgagtcgtat tggtaccggt agaaaaaatg agtaaaggag
     1201 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     1261 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     1321 aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac atatatatac
     1381 taactaaccc tgattattta aattttcagc caacacttgt cactactttc tgttatggtg
     1441 ttcaatgctt ctcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
     1501 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     1561 cacgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcaggtgctg
     1621 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     1681 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     1741 acatcatggc agacaaacaa aagaatggaa tcaaagttgt aagtttaaac atgattttac
     1801 taactaacta atctgattta aattttcaga acttcaaaat tagacacaac attgaagatg
     1861 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     1921 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
     1981 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
     2041 acgaactata caaagcaagt ttgtacaaaa aagttgaacg agaaacgtaa aatgatataa
     2101 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
     2161 aacatatgca gtcactatga atcaactact tagatggtat tagtgacctg tagaattcga
     2221 gctctagagc tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta catggtcata
     2281 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2341 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2401 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2461 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2521 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2581 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2641 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2701 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2761 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2821 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2881 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2941 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3001 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3061 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3121 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3181 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3241 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3301 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3361 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3421 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3481 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3541 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3601 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3661 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3721 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     3781 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3841 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3901 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3961 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
